Rice scientists use blood test to track gene expression in the brain

The brain is the most protected organ in the body, sheathed in a complex and nearly impenetrable barrier of specialized blood vessels. While this particular anatomical setup protects it from outside invaders, it also makes it difficult for researchers to study how specific genes are expressed ⎯ and how such changes in gene expression may lead to disease.

Now Rice University scientists have developed a noninvasive way to monitor gene expression dynamics in the brain, making it easier to investigate brain development, cognitive function and neurological diseases, according to a study published in Nature Biotechnology.

Rice bioengineer Jerzy Szablowski and colleagues have engineered a unique class of molecules, known as released markers of activity (RMAs), that can be used to measure gene expression in the brain through a simple blood test.

Typically, if you wanted to look at gene expression in the brain, you would have to wait to do a post-mortem analysis. There are some more modern neuroimaging techniques that can do this, but they lack sensitivity and specificity to track changes in specific cell types. With the RMA platform, we can introduce a synthetic gene expression reporter to the brain, which produces a protein that can pass through the blood-brain barrier. We can then measure changes in expression for a gene of interest with a simple blood test.

Jerzy Szablowski, an assistant professor of bioengineering at Rice’s George R. Brown School of Engineering.

For now, Szablowski sees RMAs as a vital research tool to help scientists better monitor gene expression in the brain. For example, he said, the RMA platform could be used to look at how long novel gene therapies stay the brain over time.

We could track these new therapies with just a blood test and continue to monitor them over time since the RMA platform is noninvasive. But we can also use RMAs to study gene expression as it relates to disease. Being able to track different gene expression changes will allow us to understand what leads to disease and how the disease itself changes gene expression in the brain. This could provide new clues for drug development, or even for how to prevent neurological diseases in the first place.

Jerzy Szablowski

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